College of Veterinary Science > Animal reproduction gynecology & obstetrics > Achivements

Artificial insemination in goat using cryopreserved semen

Newer techniques of processing and freezing of goat semen which was otherwise poorly freezable were evolved and commercialized. A centrifugation regime is standardised and a commercial bovine semen extender (Optixcell) was used for cryopreservation of goat semen and found effective as compared to Tris and Bioxcell extender. Artificial inseminations are performed at field level using cryopreserved semen and different doses of semen are given to ALDA.

Transferring Artificial Insemination in swine at field level

Artificial insemination allows small scale raiser to keep a few sows without maintaining a boar; eliminates the problem of mating boars and sows of different sizes; increases the number of sows bred by a boar and the possibility of extending the boars’ productive life and reduces breeding cost. Fresh semen was collected from Hampshire boar, processed and liquid semen was preserved at 15°C. Artificial insemination was performed in swine using liquid boar semen in field level for genetic improvement of the pig population of Assam.

Cryopreservation of buffalo semen

Buffalo semen were collected and cryopreserved using TRIS extender with glycerol and artificial insemination was done successfully.

Therapeutic management of bovine infertility

Due to faulty management cow used to suffer from different metabolic or infectious diseases which results in infertility in cow. Which affect the production and reproduction in cow with a negative impact on the economy of the farmer. Various infertility cum treatment camps were conducted in different districts of the state.Hormonal interventions viz., Ovsynch, Ovsynch plus, Double synch and Heatsynchand supplementation of feed additives (mineral mixture, bypass protein, herbal anti stressor, selenium and vitamin E) were efficaciously used to treat postpartum anestrus cow.

Some of the therapeutic protocols are explained below:

· 1)A total of 85 non- infectious repeat breeding crossbred cows were fed with 30 gm mineral mixture per animal per day for 30 days, 200 gm bypass fat per animal per day for 30 days, vitamin A, D & E suspension 20 ml per animal per day for 15 days in locally available feed followed by intramuscular administration of 15 ml meloxicam (a cox2 inhibitor) on day 16 and 17 post AI. Out of 85 non- infectious repeat breeding crossbred cows with supplement and meloxicam injection, 67 cows (78.82 %) were considered to be pregnant on non-return basis and 31 (46.27 %) cows were found to have conceived on 1st A.I.

2) A total of 147 postpartum anestrus cows were supplemented with 30 g of Mineral Mixture, 10 g Bypass Protein and 10 ml Selenium&Vitamin E for 20, 10 and 10 days respectively in concentrate feed on 147 crossbred post-partum anoestrous cows in field condition.Out of 147 post-partum anoestrous cows 104 (70.75 %) responded to treatment of which 62 (59.62 %) did not return to oestrus on first A.I

3) A total of 42 infectious repeat breeder cows were treated with Lugol’s iodine (0.25%) solution along with the supportive therapy which includes a) Bypass Fat (Fatomax, Intas Pharmaceuticals Limited) @ 150gm per day per animal with concentrate mixture for 20 days; b) Mineral mixture (Minfa Gold, Intas Pharmaceuticals Limited) @ 30gm daily per animal with concentrate mixture for a period of 30 days; c) Injectable Phosphorus (Tonophosphan Vet, MSD-Animal Health, Pune, India)2 gm intramuscularly per day for three occasions at alternate days; d) Vitamin A (Intavita H, Intas Pharmaceuticals limited) @ 5ml intramuscularly for three occasions at alternate days which gives the best result in terms of conception.

In-vitro fertilization in pig:

For the first time in India, 8 cell and 4 cell pig embryos were developed through in-vitro maturation and fertilization in collaboration with ICAR RC for NEH Region, Umiam, Meghalaya.


Publication Type Number
Research Article 276
Books 4
Book Chapter 37
Thesis 29